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    Labconco refrigerated centrivap vacuum concentrators labconco
    Refrigerated Centrivap Vacuum Concentrators Labconco, supplied by Labconco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/refrigerated+centrivap+vacuum+concentrators+labconco/pm38041820-56-141-145
    Average 86 stars, based on 1 article reviews
    refrigerated centrivap vacuum concentrators labconco - by Bioz Stars, 2026-10
    86/100 stars

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    Saline:

    Article Title: Protocol for low-input proteomic analysis of in situ fixed adult murine muscle stem cells.
    Article Snippet: GENT or RESOURCE SOURCE IDENTIFIER icals, peptides, and recombinant proteins Paraformaldehyde (PFA) aqueous ion, EM grade Electron Microscopy Sciences Catalog #: 15714 onitrile (ACN) Honeywell Catalog #: 34967 one puriss. p.a., ACS reagent, .5% (GC) Honeywell Catalog #: 32201 genase, type 2 Worthington Biochemical Catalog #: LS004177 ase II, powder Thermo Fisher Scientific Catalog #: 17105041 othreitol (DTT) USB Corporation Catalog #: 3483-12-3 nol absolute VWR Chemicals Catalog #: 20821.330 ic acid Thermo Fisher Scientific Catalog #: 85178 ine, for electrophoresis, R99% Sigma-Aldrich Catalog #: G8898 ’s F-10 nutrient mix Thermo Fisher Scientific Catalog #: 11550043 e serum (HS) Invitrogen Catalog #: 16050114 acetamide (IAA) Sigma-Aldrich Catalog #: l1149-25G ion molecular grade ethylenediaminetetraacetic (EDTA) (0.5 M), pH 8.0 Thermo Fisher Scientific Catalog #: AM9260G (Continued on next page) STAR Protocols 4, 102750, December 15, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Pentobarbital sodium (Dorminal 20% inj.) .. Alfamedic Catalog #: 013003 Phosphate-buffered saline (PBS) Sigma-Aldrich Catalog #: P3813 Penicillin-Streptomycin (P/S) (10,000 U/mL) Thermo Fisher Scientific Catalog #: 15140122 Sodium chloride (NaCl) Sigma-Aldrich Catalog #: 31434 Sodium dodecyl sulfate (SDS) Sigma-Aldrich Catalog #: L3771-500G Sodium deoxycholate Sigma-Aldrich Catalog #: 89904 Trifluoroacetic acid Sigma-Aldrich Catalog #: T6508 Triton X-100, laboratory grade Sigma-Aldrich Catalog #: X100-500ML Trizma base, primary standard and buffer, >=99.9% (titration), crystalline Sigma-Aldrich Catalog #: T1503-100G UltraPure DNase/RNase-free distilled water Thermo Fisher Scientific Catalog #: 10977015 Critical commercial assays iST kit 963 PreOmics Catalog #: P.O.00027 BCA protein assay kit Thermo Fisher Scientific Catalog #: 23225 Experimental models: Organisms/strains Mouse: ROSA26YFP/YFP (male, 2–4 months old) JAX JAX: 006148 Software and algorithms PEAKS software (Version: Xpro) Bioinformatics Solutions, Inc. N/A Other 20 mL syringe Terumo Catalog #: SS+20L 40 mm cell strainer SPL Life Sciences Catalog #: 93040 Acid-resistant refrigerated CentriVap vacuum concentrators Labconco Catalog #: 7310034 BD Influx cell sorter BD Biosciences N/A CentriVap –105 C cold trap Labconco Catalog #: 7385035 C18 column IonOpticks Catalog #: AUR2-25075C18A-CSI Disposable needle 20 G 3 11/2 inch Terumo Catalog #: NN+2038R Eppendorf centrifuge 5427R Eppendorf Catalog #: 5427R Eppendorf centrifuge 5804R Eppendorf Catalog #: 5804R 50 mL high clarity PP centrifuge tube Falcon Catalog #: 352070 Filter upper cup, PES 0.22 mm, 500 mL, 75 mm Jet Bio-filtration Catalog #: FPE214150 MD 4C NT chemistry diaphragm vacuum pump Vacuubrand Catalog #: 7393001 Precise peristaltic pump Longer Precision Pump Catalog #: BT100-2J Round-bottom polypropylene tube, 5 mL Falcon Catalog #: 352063 Round-bottom polystyrene test tube, with cell strainer snap cap, 5 mL Falcon Catalog #: 352235 Syringe filter, Minisart high flow, 0.22 mm, PES membrane, sterile, 28 mm Sartorius Catalog #: 1209Z85 SURFLO winged infusion set Terumo Catalog #: SV*23NL Shaking water bath Memmert Catalog #: WNB 22 timsTOF Pro mass spectrometer or other equivalent low-input mass spectrometer Bruker or others N/A ll OPEN ACCESSProtocol ..

    Titration:

    Article Title: Protocol for low-input proteomic analysis of in situ fixed adult murine muscle stem cells.
    Article Snippet: GENT or RESOURCE SOURCE IDENTIFIER icals, peptides, and recombinant proteins Paraformaldehyde (PFA) aqueous ion, EM grade Electron Microscopy Sciences Catalog #: 15714 onitrile (ACN) Honeywell Catalog #: 34967 one puriss. p.a., ACS reagent, .5% (GC) Honeywell Catalog #: 32201 genase, type 2 Worthington Biochemical Catalog #: LS004177 ase II, powder Thermo Fisher Scientific Catalog #: 17105041 othreitol (DTT) USB Corporation Catalog #: 3483-12-3 nol absolute VWR Chemicals Catalog #: 20821.330 ic acid Thermo Fisher Scientific Catalog #: 85178 ine, for electrophoresis, R99% Sigma-Aldrich Catalog #: G8898 ’s F-10 nutrient mix Thermo Fisher Scientific Catalog #: 11550043 e serum (HS) Invitrogen Catalog #: 16050114 acetamide (IAA) Sigma-Aldrich Catalog #: l1149-25G ion molecular grade ethylenediaminetetraacetic (EDTA) (0.5 M), pH 8.0 Thermo Fisher Scientific Catalog #: AM9260G (Continued on next page) STAR Protocols 4, 102750, December 15, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Pentobarbital sodium (Dorminal 20% inj.) .. Alfamedic Catalog #: 013003 Phosphate-buffered saline (PBS) Sigma-Aldrich Catalog #: P3813 Penicillin-Streptomycin (P/S) (10,000 U/mL) Thermo Fisher Scientific Catalog #: 15140122 Sodium chloride (NaCl) Sigma-Aldrich Catalog #: 31434 Sodium dodecyl sulfate (SDS) Sigma-Aldrich Catalog #: L3771-500G Sodium deoxycholate Sigma-Aldrich Catalog #: 89904 Trifluoroacetic acid Sigma-Aldrich Catalog #: T6508 Triton X-100, laboratory grade Sigma-Aldrich Catalog #: X100-500ML Trizma base, primary standard and buffer, >=99.9% (titration), crystalline Sigma-Aldrich Catalog #: T1503-100G UltraPure DNase/RNase-free distilled water Thermo Fisher Scientific Catalog #: 10977015 Critical commercial assays iST kit 963 PreOmics Catalog #: P.O.00027 BCA protein assay kit Thermo Fisher Scientific Catalog #: 23225 Experimental models: Organisms/strains Mouse: ROSA26YFP/YFP (male, 2–4 months old) JAX JAX: 006148 Software and algorithms PEAKS software (Version: Xpro) Bioinformatics Solutions, Inc. N/A Other 20 mL syringe Terumo Catalog #: SS+20L 40 mm cell strainer SPL Life Sciences Catalog #: 93040 Acid-resistant refrigerated CentriVap vacuum concentrators Labconco Catalog #: 7310034 BD Influx cell sorter BD Biosciences N/A CentriVap –105 C cold trap Labconco Catalog #: 7385035 C18 column IonOpticks Catalog #: AUR2-25075C18A-CSI Disposable needle 20 G 3 11/2 inch Terumo Catalog #: NN+2038R Eppendorf centrifuge 5427R Eppendorf Catalog #: 5427R Eppendorf centrifuge 5804R Eppendorf Catalog #: 5804R 50 mL high clarity PP centrifuge tube Falcon Catalog #: 352070 Filter upper cup, PES 0.22 mm, 500 mL, 75 mm Jet Bio-filtration Catalog #: FPE214150 MD 4C NT chemistry diaphragm vacuum pump Vacuubrand Catalog #: 7393001 Precise peristaltic pump Longer Precision Pump Catalog #: BT100-2J Round-bottom polypropylene tube, 5 mL Falcon Catalog #: 352063 Round-bottom polystyrene test tube, with cell strainer snap cap, 5 mL Falcon Catalog #: 352235 Syringe filter, Minisart high flow, 0.22 mm, PES membrane, sterile, 28 mm Sartorius Catalog #: 1209Z85 SURFLO winged infusion set Terumo Catalog #: SV*23NL Shaking water bath Memmert Catalog #: WNB 22 timsTOF Pro mass spectrometer or other equivalent low-input mass spectrometer Bruker or others N/A ll OPEN ACCESSProtocol ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Protocol for low-input proteomic analysis of in situ fixed adult murine muscle stem cells.
    Article Snippet: GENT or RESOURCE SOURCE IDENTIFIER icals, peptides, and recombinant proteins Paraformaldehyde (PFA) aqueous ion, EM grade Electron Microscopy Sciences Catalog #: 15714 onitrile (ACN) Honeywell Catalog #: 34967 one puriss. p.a., ACS reagent, .5% (GC) Honeywell Catalog #: 32201 genase, type 2 Worthington Biochemical Catalog #: LS004177 ase II, powder Thermo Fisher Scientific Catalog #: 17105041 othreitol (DTT) USB Corporation Catalog #: 3483-12-3 nol absolute VWR Chemicals Catalog #: 20821.330 ic acid Thermo Fisher Scientific Catalog #: 85178 ine, for electrophoresis, R99% Sigma-Aldrich Catalog #: G8898 ’s F-10 nutrient mix Thermo Fisher Scientific Catalog #: 11550043 e serum (HS) Invitrogen Catalog #: 16050114 acetamide (IAA) Sigma-Aldrich Catalog #: l1149-25G ion molecular grade ethylenediaminetetraacetic (EDTA) (0.5 M), pH 8.0 Thermo Fisher Scientific Catalog #: AM9260G (Continued on next page) STAR Protocols 4, 102750, December 15, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Pentobarbital sodium (Dorminal 20% inj.) .. Alfamedic Catalog #: 013003 Phosphate-buffered saline (PBS) Sigma-Aldrich Catalog #: P3813 Penicillin-Streptomycin (P/S) (10,000 U/mL) Thermo Fisher Scientific Catalog #: 15140122 Sodium chloride (NaCl) Sigma-Aldrich Catalog #: 31434 Sodium dodecyl sulfate (SDS) Sigma-Aldrich Catalog #: L3771-500G Sodium deoxycholate Sigma-Aldrich Catalog #: 89904 Trifluoroacetic acid Sigma-Aldrich Catalog #: T6508 Triton X-100, laboratory grade Sigma-Aldrich Catalog #: X100-500ML Trizma base, primary standard and buffer, >=99.9% (titration), crystalline Sigma-Aldrich Catalog #: T1503-100G UltraPure DNase/RNase-free distilled water Thermo Fisher Scientific Catalog #: 10977015 Critical commercial assays iST kit 963 PreOmics Catalog #: P.O.00027 BCA protein assay kit Thermo Fisher Scientific Catalog #: 23225 Experimental models: Organisms/strains Mouse: ROSA26YFP/YFP (male, 2–4 months old) JAX JAX: 006148 Software and algorithms PEAKS software (Version: Xpro) Bioinformatics Solutions, Inc. N/A Other 20 mL syringe Terumo Catalog #: SS+20L 40 mm cell strainer SPL Life Sciences Catalog #: 93040 Acid-resistant refrigerated CentriVap vacuum concentrators Labconco Catalog #: 7310034 BD Influx cell sorter BD Biosciences N/A CentriVap –105 C cold trap Labconco Catalog #: 7385035 C18 column IonOpticks Catalog #: AUR2-25075C18A-CSI Disposable needle 20 G 3 11/2 inch Terumo Catalog #: NN+2038R Eppendorf centrifuge 5427R Eppendorf Catalog #: 5427R Eppendorf centrifuge 5804R Eppendorf Catalog #: 5804R 50 mL high clarity PP centrifuge tube Falcon Catalog #: 352070 Filter upper cup, PES 0.22 mm, 500 mL, 75 mm Jet Bio-filtration Catalog #: FPE214150 MD 4C NT chemistry diaphragm vacuum pump Vacuubrand Catalog #: 7393001 Precise peristaltic pump Longer Precision Pump Catalog #: BT100-2J Round-bottom polypropylene tube, 5 mL Falcon Catalog #: 352063 Round-bottom polystyrene test tube, with cell strainer snap cap, 5 mL Falcon Catalog #: 352235 Syringe filter, Minisart high flow, 0.22 mm, PES membrane, sterile, 28 mm Sartorius Catalog #: 1209Z85 SURFLO winged infusion set Terumo Catalog #: SV*23NL Shaking water bath Memmert Catalog #: WNB 22 timsTOF Pro mass spectrometer or other equivalent low-input mass spectrometer Bruker or others N/A ll OPEN ACCESSProtocol ..

    Software:

    Article Title: Protocol for low-input proteomic analysis of in situ fixed adult murine muscle stem cells.
    Article Snippet: GENT or RESOURCE SOURCE IDENTIFIER icals, peptides, and recombinant proteins Paraformaldehyde (PFA) aqueous ion, EM grade Electron Microscopy Sciences Catalog #: 15714 onitrile (ACN) Honeywell Catalog #: 34967 one puriss. p.a., ACS reagent, .5% (GC) Honeywell Catalog #: 32201 genase, type 2 Worthington Biochemical Catalog #: LS004177 ase II, powder Thermo Fisher Scientific Catalog #: 17105041 othreitol (DTT) USB Corporation Catalog #: 3483-12-3 nol absolute VWR Chemicals Catalog #: 20821.330 ic acid Thermo Fisher Scientific Catalog #: 85178 ine, for electrophoresis, R99% Sigma-Aldrich Catalog #: G8898 ’s F-10 nutrient mix Thermo Fisher Scientific Catalog #: 11550043 e serum (HS) Invitrogen Catalog #: 16050114 acetamide (IAA) Sigma-Aldrich Catalog #: l1149-25G ion molecular grade ethylenediaminetetraacetic (EDTA) (0.5 M), pH 8.0 Thermo Fisher Scientific Catalog #: AM9260G (Continued on next page) STAR Protocols 4, 102750, December 15, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Pentobarbital sodium (Dorminal 20% inj.) .. Alfamedic Catalog #: 013003 Phosphate-buffered saline (PBS) Sigma-Aldrich Catalog #: P3813 Penicillin-Streptomycin (P/S) (10,000 U/mL) Thermo Fisher Scientific Catalog #: 15140122 Sodium chloride (NaCl) Sigma-Aldrich Catalog #: 31434 Sodium dodecyl sulfate (SDS) Sigma-Aldrich Catalog #: L3771-500G Sodium deoxycholate Sigma-Aldrich Catalog #: 89904 Trifluoroacetic acid Sigma-Aldrich Catalog #: T6508 Triton X-100, laboratory grade Sigma-Aldrich Catalog #: X100-500ML Trizma base, primary standard and buffer, >=99.9% (titration), crystalline Sigma-Aldrich Catalog #: T1503-100G UltraPure DNase/RNase-free distilled water Thermo Fisher Scientific Catalog #: 10977015 Critical commercial assays iST kit 963 PreOmics Catalog #: P.O.00027 BCA protein assay kit Thermo Fisher Scientific Catalog #: 23225 Experimental models: Organisms/strains Mouse: ROSA26YFP/YFP (male, 2–4 months old) JAX JAX: 006148 Software and algorithms PEAKS software (Version: Xpro) Bioinformatics Solutions, Inc. N/A Other 20 mL syringe Terumo Catalog #: SS+20L 40 mm cell strainer SPL Life Sciences Catalog #: 93040 Acid-resistant refrigerated CentriVap vacuum concentrators Labconco Catalog #: 7310034 BD Influx cell sorter BD Biosciences N/A CentriVap –105 C cold trap Labconco Catalog #: 7385035 C18 column IonOpticks Catalog #: AUR2-25075C18A-CSI Disposable needle 20 G 3 11/2 inch Terumo Catalog #: NN+2038R Eppendorf centrifuge 5427R Eppendorf Catalog #: 5427R Eppendorf centrifuge 5804R Eppendorf Catalog #: 5804R 50 mL high clarity PP centrifuge tube Falcon Catalog #: 352070 Filter upper cup, PES 0.22 mm, 500 mL, 75 mm Jet Bio-filtration Catalog #: FPE214150 MD 4C NT chemistry diaphragm vacuum pump Vacuubrand Catalog #: 7393001 Precise peristaltic pump Longer Precision Pump Catalog #: BT100-2J Round-bottom polypropylene tube, 5 mL Falcon Catalog #: 352063 Round-bottom polystyrene test tube, with cell strainer snap cap, 5 mL Falcon Catalog #: 352235 Syringe filter, Minisart high flow, 0.22 mm, PES membrane, sterile, 28 mm Sartorius Catalog #: 1209Z85 SURFLO winged infusion set Terumo Catalog #: SV*23NL Shaking water bath Memmert Catalog #: WNB 22 timsTOF Pro mass spectrometer or other equivalent low-input mass spectrometer Bruker or others N/A ll OPEN ACCESSProtocol ..

    Membrane:

    Article Title: Protocol for low-input proteomic analysis of in situ fixed adult murine muscle stem cells.
    Article Snippet: GENT or RESOURCE SOURCE IDENTIFIER icals, peptides, and recombinant proteins Paraformaldehyde (PFA) aqueous ion, EM grade Electron Microscopy Sciences Catalog #: 15714 onitrile (ACN) Honeywell Catalog #: 34967 one puriss. p.a., ACS reagent, .5% (GC) Honeywell Catalog #: 32201 genase, type 2 Worthington Biochemical Catalog #: LS004177 ase II, powder Thermo Fisher Scientific Catalog #: 17105041 othreitol (DTT) USB Corporation Catalog #: 3483-12-3 nol absolute VWR Chemicals Catalog #: 20821.330 ic acid Thermo Fisher Scientific Catalog #: 85178 ine, for electrophoresis, R99% Sigma-Aldrich Catalog #: G8898 ’s F-10 nutrient mix Thermo Fisher Scientific Catalog #: 11550043 e serum (HS) Invitrogen Catalog #: 16050114 acetamide (IAA) Sigma-Aldrich Catalog #: l1149-25G ion molecular grade ethylenediaminetetraacetic (EDTA) (0.5 M), pH 8.0 Thermo Fisher Scientific Catalog #: AM9260G (Continued on next page) STAR Protocols 4, 102750, December 15, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Pentobarbital sodium (Dorminal 20% inj.) .. Alfamedic Catalog #: 013003 Phosphate-buffered saline (PBS) Sigma-Aldrich Catalog #: P3813 Penicillin-Streptomycin (P/S) (10,000 U/mL) Thermo Fisher Scientific Catalog #: 15140122 Sodium chloride (NaCl) Sigma-Aldrich Catalog #: 31434 Sodium dodecyl sulfate (SDS) Sigma-Aldrich Catalog #: L3771-500G Sodium deoxycholate Sigma-Aldrich Catalog #: 89904 Trifluoroacetic acid Sigma-Aldrich Catalog #: T6508 Triton X-100, laboratory grade Sigma-Aldrich Catalog #: X100-500ML Trizma base, primary standard and buffer, >=99.9% (titration), crystalline Sigma-Aldrich Catalog #: T1503-100G UltraPure DNase/RNase-free distilled water Thermo Fisher Scientific Catalog #: 10977015 Critical commercial assays iST kit 963 PreOmics Catalog #: P.O.00027 BCA protein assay kit Thermo Fisher Scientific Catalog #: 23225 Experimental models: Organisms/strains Mouse: ROSA26YFP/YFP (male, 2–4 months old) JAX JAX: 006148 Software and algorithms PEAKS software (Version: Xpro) Bioinformatics Solutions, Inc. N/A Other 20 mL syringe Terumo Catalog #: SS+20L 40 mm cell strainer SPL Life Sciences Catalog #: 93040 Acid-resistant refrigerated CentriVap vacuum concentrators Labconco Catalog #: 7310034 BD Influx cell sorter BD Biosciences N/A CentriVap –105 C cold trap Labconco Catalog #: 7385035 C18 column IonOpticks Catalog #: AUR2-25075C18A-CSI Disposable needle 20 G 3 11/2 inch Terumo Catalog #: NN+2038R Eppendorf centrifuge 5427R Eppendorf Catalog #: 5427R Eppendorf centrifuge 5804R Eppendorf Catalog #: 5804R 50 mL high clarity PP centrifuge tube Falcon Catalog #: 352070 Filter upper cup, PES 0.22 mm, 500 mL, 75 mm Jet Bio-filtration Catalog #: FPE214150 MD 4C NT chemistry diaphragm vacuum pump Vacuubrand Catalog #: 7393001 Precise peristaltic pump Longer Precision Pump Catalog #: BT100-2J Round-bottom polypropylene tube, 5 mL Falcon Catalog #: 352063 Round-bottom polystyrene test tube, with cell strainer snap cap, 5 mL Falcon Catalog #: 352235 Syringe filter, Minisart high flow, 0.22 mm, PES membrane, sterile, 28 mm Sartorius Catalog #: 1209Z85 SURFLO winged infusion set Terumo Catalog #: SV*23NL Shaking water bath Memmert Catalog #: WNB 22 timsTOF Pro mass spectrometer or other equivalent low-input mass spectrometer Bruker or others N/A ll OPEN ACCESSProtocol ..

    Sterility:

    Article Title: Protocol for low-input proteomic analysis of in situ fixed adult murine muscle stem cells.
    Article Snippet: GENT or RESOURCE SOURCE IDENTIFIER icals, peptides, and recombinant proteins Paraformaldehyde (PFA) aqueous ion, EM grade Electron Microscopy Sciences Catalog #: 15714 onitrile (ACN) Honeywell Catalog #: 34967 one puriss. p.a., ACS reagent, .5% (GC) Honeywell Catalog #: 32201 genase, type 2 Worthington Biochemical Catalog #: LS004177 ase II, powder Thermo Fisher Scientific Catalog #: 17105041 othreitol (DTT) USB Corporation Catalog #: 3483-12-3 nol absolute VWR Chemicals Catalog #: 20821.330 ic acid Thermo Fisher Scientific Catalog #: 85178 ine, for electrophoresis, R99% Sigma-Aldrich Catalog #: G8898 ’s F-10 nutrient mix Thermo Fisher Scientific Catalog #: 11550043 e serum (HS) Invitrogen Catalog #: 16050114 acetamide (IAA) Sigma-Aldrich Catalog #: l1149-25G ion molecular grade ethylenediaminetetraacetic (EDTA) (0.5 M), pH 8.0 Thermo Fisher Scientific Catalog #: AM9260G (Continued on next page) STAR Protocols 4, 102750, December 15, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Pentobarbital sodium (Dorminal 20% inj.) .. Alfamedic Catalog #: 013003 Phosphate-buffered saline (PBS) Sigma-Aldrich Catalog #: P3813 Penicillin-Streptomycin (P/S) (10,000 U/mL) Thermo Fisher Scientific Catalog #: 15140122 Sodium chloride (NaCl) Sigma-Aldrich Catalog #: 31434 Sodium dodecyl sulfate (SDS) Sigma-Aldrich Catalog #: L3771-500G Sodium deoxycholate Sigma-Aldrich Catalog #: 89904 Trifluoroacetic acid Sigma-Aldrich Catalog #: T6508 Triton X-100, laboratory grade Sigma-Aldrich Catalog #: X100-500ML Trizma base, primary standard and buffer, >=99.9% (titration), crystalline Sigma-Aldrich Catalog #: T1503-100G UltraPure DNase/RNase-free distilled water Thermo Fisher Scientific Catalog #: 10977015 Critical commercial assays iST kit 963 PreOmics Catalog #: P.O.00027 BCA protein assay kit Thermo Fisher Scientific Catalog #: 23225 Experimental models: Organisms/strains Mouse: ROSA26YFP/YFP (male, 2–4 months old) JAX JAX: 006148 Software and algorithms PEAKS software (Version: Xpro) Bioinformatics Solutions, Inc. N/A Other 20 mL syringe Terumo Catalog #: SS+20L 40 mm cell strainer SPL Life Sciences Catalog #: 93040 Acid-resistant refrigerated CentriVap vacuum concentrators Labconco Catalog #: 7310034 BD Influx cell sorter BD Biosciences N/A CentriVap –105 C cold trap Labconco Catalog #: 7385035 C18 column IonOpticks Catalog #: AUR2-25075C18A-CSI Disposable needle 20 G 3 11/2 inch Terumo Catalog #: NN+2038R Eppendorf centrifuge 5427R Eppendorf Catalog #: 5427R Eppendorf centrifuge 5804R Eppendorf Catalog #: 5804R 50 mL high clarity PP centrifuge tube Falcon Catalog #: 352070 Filter upper cup, PES 0.22 mm, 500 mL, 75 mm Jet Bio-filtration Catalog #: FPE214150 MD 4C NT chemistry diaphragm vacuum pump Vacuubrand Catalog #: 7393001 Precise peristaltic pump Longer Precision Pump Catalog #: BT100-2J Round-bottom polypropylene tube, 5 mL Falcon Catalog #: 352063 Round-bottom polystyrene test tube, with cell strainer snap cap, 5 mL Falcon Catalog #: 352235 Syringe filter, Minisart high flow, 0.22 mm, PES membrane, sterile, 28 mm Sartorius Catalog #: 1209Z85 SURFLO winged infusion set Terumo Catalog #: SV*23NL Shaking water bath Memmert Catalog #: WNB 22 timsTOF Pro mass spectrometer or other equivalent low-input mass spectrometer Bruker or others N/A ll OPEN ACCESSProtocol ..

    Mass Spectrometry:

    Article Title: Protocol for low-input proteomic analysis of in situ fixed adult murine muscle stem cells.
    Article Snippet: GENT or RESOURCE SOURCE IDENTIFIER icals, peptides, and recombinant proteins Paraformaldehyde (PFA) aqueous ion, EM grade Electron Microscopy Sciences Catalog #: 15714 onitrile (ACN) Honeywell Catalog #: 34967 one puriss. p.a., ACS reagent, .5% (GC) Honeywell Catalog #: 32201 genase, type 2 Worthington Biochemical Catalog #: LS004177 ase II, powder Thermo Fisher Scientific Catalog #: 17105041 othreitol (DTT) USB Corporation Catalog #: 3483-12-3 nol absolute VWR Chemicals Catalog #: 20821.330 ic acid Thermo Fisher Scientific Catalog #: 85178 ine, for electrophoresis, R99% Sigma-Aldrich Catalog #: G8898 ’s F-10 nutrient mix Thermo Fisher Scientific Catalog #: 11550043 e serum (HS) Invitrogen Catalog #: 16050114 acetamide (IAA) Sigma-Aldrich Catalog #: l1149-25G ion molecular grade ethylenediaminetetraacetic (EDTA) (0.5 M), pH 8.0 Thermo Fisher Scientific Catalog #: AM9260G (Continued on next page) STAR Protocols 4, 102750, December 15, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Pentobarbital sodium (Dorminal 20% inj.) .. Alfamedic Catalog #: 013003 Phosphate-buffered saline (PBS) Sigma-Aldrich Catalog #: P3813 Penicillin-Streptomycin (P/S) (10,000 U/mL) Thermo Fisher Scientific Catalog #: 15140122 Sodium chloride (NaCl) Sigma-Aldrich Catalog #: 31434 Sodium dodecyl sulfate (SDS) Sigma-Aldrich Catalog #: L3771-500G Sodium deoxycholate Sigma-Aldrich Catalog #: 89904 Trifluoroacetic acid Sigma-Aldrich Catalog #: T6508 Triton X-100, laboratory grade Sigma-Aldrich Catalog #: X100-500ML Trizma base, primary standard and buffer, >=99.9% (titration), crystalline Sigma-Aldrich Catalog #: T1503-100G UltraPure DNase/RNase-free distilled water Thermo Fisher Scientific Catalog #: 10977015 Critical commercial assays iST kit 963 PreOmics Catalog #: P.O.00027 BCA protein assay kit Thermo Fisher Scientific Catalog #: 23225 Experimental models: Organisms/strains Mouse: ROSA26YFP/YFP (male, 2–4 months old) JAX JAX: 006148 Software and algorithms PEAKS software (Version: Xpro) Bioinformatics Solutions, Inc. N/A Other 20 mL syringe Terumo Catalog #: SS+20L 40 mm cell strainer SPL Life Sciences Catalog #: 93040 Acid-resistant refrigerated CentriVap vacuum concentrators Labconco Catalog #: 7310034 BD Influx cell sorter BD Biosciences N/A CentriVap –105 C cold trap Labconco Catalog #: 7385035 C18 column IonOpticks Catalog #: AUR2-25075C18A-CSI Disposable needle 20 G 3 11/2 inch Terumo Catalog #: NN+2038R Eppendorf centrifuge 5427R Eppendorf Catalog #: 5427R Eppendorf centrifuge 5804R Eppendorf Catalog #: 5804R 50 mL high clarity PP centrifuge tube Falcon Catalog #: 352070 Filter upper cup, PES 0.22 mm, 500 mL, 75 mm Jet Bio-filtration Catalog #: FPE214150 MD 4C NT chemistry diaphragm vacuum pump Vacuubrand Catalog #: 7393001 Precise peristaltic pump Longer Precision Pump Catalog #: BT100-2J Round-bottom polypropylene tube, 5 mL Falcon Catalog #: 352063 Round-bottom polystyrene test tube, with cell strainer snap cap, 5 mL Falcon Catalog #: 352235 Syringe filter, Minisart high flow, 0.22 mm, PES membrane, sterile, 28 mm Sartorius Catalog #: 1209Z85 SURFLO winged infusion set Terumo Catalog #: SV*23NL Shaking water bath Memmert Catalog #: WNB 22 timsTOF Pro mass spectrometer or other equivalent low-input mass spectrometer Bruker or others N/A ll OPEN ACCESSProtocol ..



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